Examples demonstrating the estimates from rSeqDiff.

Abstract

<p>(A)-(C) show NRCAM gene. (D)–(F) show BACE1 gene. (G)-(I) show SCIN gene. (A)(D)(G) show the gene structure and coverage of reads mapped to the gene. (B)(E)(H) show enlargement of the parts in the red boxes in (A)(D)(G), respectively, emphasizing the alternative spliced exons. In (B), the red box emphasizes the alternative exon that was validated by RT-PCR assay in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079448#pone.0079448-Voineagu1" target="_blank">[19]</a>, and the two red arrows represent the positions of the primers of RT-PCR <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079448#pone.0079448-Voineagu1" target="_blank">[19]</a>. (C)(F)(I) show estimated abundances for each gene and its isoforms by rSeqDiff. Values in the brackets are the 95% confidence intervals for the estimates.</p