<p>(A) Schematic diagram showing generation of the recombinant <i>hCYP2J2</i> BAC. Two targeting vectors were constructed to remove the sequences flanking <i>hCYP2J2</i> by homologous recombination in <i>E. coli</i>. Primers P15 to 22 were used to identify the recombinants in E. coli. PCR data are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003950#pgen.1003950.s005" target="_blank">Figure S5</a>. Trpr∧r, trimethoprim resistance; Amp∧r, Ampicillin resistance; Hyg, Hygromycin. (B). Transgenic mice were identified by PCR and confirmed by DNA blotting. M, λ DNA marker/<i>Hin</i>d III; −, negative PCR control; +, positive PCR control to amplify BAC; 1 to 5, 8, founder mice.</p
