Maspin nuclear localization correlates with increased histone acetylation and release of HDAC-repressed gene expression.

Abstract

<p>(<b>A</b>) Western blot of recombinant maspin, HDAC1, and HDAC1 target protein (acetylated Histone 3 at Lysine 9 (H3 Acetyl K⁹)) in DU145 cells. GAPDH was used as a loading control. (<b>B</b>) Q-RT-PCR of HDAC1 targeted genes differentially regulated by maspin. The threshold cycle (ct) numbers obtained from qRT-PCR were normalized by the internal GAPDH controls and presented as the fold change. Maspin^WT: black bar; maspin^D346E: white bar.</p