Validation of mature miR expression.
- Publication date
- Publisher
Abstract
<p>(A) Schematic representation of miRs targeted to the 3'-UTR of the MDR1 mRNA predicted by <a href="http://microrna.org" target="_blank">microRNA.org</a>. MiR-381 and miR-495 are highlighted and their predicted seed binding regions are shown in (B). Numbers at both ends of the fragments represent the relative nucleotide locations in this region. (C) Expression of mature miR-381, miR-495 and miR-16 in K562 and K562/ADM cells was validated by stem-loop real-time PCR. The Ct values of these miRs were normalized to that of miR-425 whose expression was unchanged in both cell types. Next, the relative expression of miR-381, miR-495 and miR-16 in K562 cells and in K562/ADM cells was calculated. The results are shown as the mean ± SD of three independent experiments. (D) The Ct values of miR-381, miR-495 and miR-16 in K562 and K562/VBL cells were normalized to miR-425. The relative expression of miR-381, miR-495 and miR-16 in K562 cells and in K562/VBL cells was calculated. The results are shown as the mean ± SD of three independent experiments.</p