Expression of miR-30e* and Bmi1 co-cultured with M1- and M2-polarized macrophages purified from human monocytes.

Abstract

<p>(<b>A</b>) qRT-PCR analysis of miR-30e* expression in AGS cells co-cultured with M1- and M2-polarized macrophages. Significantly lower miR-30e* expression was detected in co-cultured groups compared with the control group (***<i>P</i> < 0.001, *<i>P</i> < 0.05, respectively). (<b>B</b>) qRT-PCR analysis of miR-30e* expression in HCT116 cells co-cultured with M1- and M2-polarized macrophages. Significantly lower miR-30e* expression was detected in co-cultured groups compared with the control group (***<i>P</i> < 0.001, **<i>P</i> < 0.01, respectively). (<b>C</b>) qRT-PCR analysis of Bmi1 expression in AGS cells co-cultured with M1- and M2-polarized macrophages. Significantly higher Bmi1 expression was detected in co-cultured groups compared with the control group (***<i>P</i> < 0.001, **<i>P</i> < 0.01, respectively). (<b>D</b>) qRT-PCR analysis of miR-30e* expression in HCT116 cells co-cultured with M1- and M2-polarized macrophages. Significantly higher Bmi1 expression was detected in M1 macrophage co-cultured groups compared with the control group (**<i>P</i> < 0.01). (<b>E</b>) Schematic representation of miR-30e*-Bmi1 signaling mediated by TAMs. </p