Vaccine-induced F-MuLV Env-specific CD4⁺ T cell responses.

Abstract

<p>CB6F1 mice were immunized twice with Ad.pIXgp70 in combination with adenoviral vectors encoding different interleukins. Three weeks after the second immunization, mice were challenged with 500 SFFU FV. Virus-specific CD4⁺ T cell responses were analyzed 3 days p.i. by tetramer staining of spleen cells. Statistically significant differences (<i>P</i> < 0.05; Kruskal-Wallis one-way analysis of variance on ranks with Dunns multiple comparison procedure) compared to unvaccinated mice (#) are indicated. Each dot represents an individual animal, horizontal lines indicate mean values. Data are results of two independent experiments with similar outcome.</p