Differential expression analysis of tags and unigenes by DGE.

Abstract

<p>The expression level for each tag and unigene is shown in the volcano plots (a, c, e) and (b, d, f) after 12 (a, b), 24 (c, d), and 48 h (e, f) of cold stress. ‘Not DETs’ indicates ‘not detected expression tags’ and ‘Not DEGs’ indicates ‘not detected expression genes’. The horizontal line indicates the log₁₀ of transcripts per million of the control (A) and the vertical line indicates the log₁₀ of transcripts per million of 12 (B), 24 (C), and 48 h (D) of treatment at 12°C. The criteria for screening differentially expressed individuals are based on FDR ≤ 0.001 and the absolute value of log₂Ratio ≥ 1.</p