c-Myc accumulation in outlet obstruction.

Abstract

<p>(A) Fold change (vs. sham) of c-Myc (Myc), Hdac3 and Ezh2 mRNAs in outlet obstruction and de-obstruction (n=6-8). (B) Inverse correlation between miR-29b and Myc mRNA level (both expressed relative to sham) in outlet obstruction and its reversal (n=6-8). The leftmost symbol represents 10 d obstruction, the rightmost symbol represents de-obstruction, and the symbols close to x=1 represent sham and 6 weeks of obstruction, respectively. Western blots and bar graph summaries showing increased c-Myc (C) and Ezh2 (D) levels in outlet obstruction (n=6 for both). (E) Western blots and bar graph summary showing increased phospho-NF-κB p105 in outlet obstruction (n=6). Blots for phospho-NF-κB p65 and IκB-α are shown without bar graph summaries as no significant differences were seen. </p