HaRxL44 is a nuclear-HaRxL that enhances plant susceptibility to <i>Hpa.</i>

Abstract

<p>(A) <i>In silico</i> prediction of HaRxL44 protein organization. SP, signal peptide; RFL, RxLR motif. (B) Monitoring of <i>Hpa</i> Waco9 sporulation at 5 d after inoculation in transgenic lines expressing HaRxL44 under the control of 35S promoter (44 lines), under the control of an “haustoriated-cell specific” promoter (dP2–44 lines), under the control of DEX inducible promoter (D44 lines). For D44 lines, plants were treated with DEX 2d after <i>Hpa</i> infection, in order to induce HaRxL44 expression. Expression of HaRxL44 in all the lines was monitored by Western blot (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001732#pbio.1001732.s003" target="_blank">Figure S3</a>). Error bars represent the standard error of the mean. Asterisks represent the significance of individual unpaired <i>t</i> tests comparing the given column with the control. (C) Subcellular localisation of GFP-HaRxL44 4 DAI with <i>Hpa</i>. The green colour corresponds to the GFP signal, and the red colour corresponds to chloroplast autofluorescence. Asterisks indicate the position of the haustorium. n, nucleus.</p

    Similar works

    Full text

    thumbnail-image

    Available Versions