HaRxL44 interacts with and destabilizes MED19a in a Proteasome-dependant manner.

Abstract

<p>(A) Schematic representation of the relevant interactions obtained by Y2H between HaRxL44 and <i>Arabidopsis</i> cDNA library. Data extracted from Mukhtar et al. (2011) <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001732#pbio.1001732-Mukhtar1" target="_blank">[17]</a>. (B) Subcellular localisation of GFP-BOI and GFP-MBR1–like determined by transient expression in <i>N. benthamiana.</i> (C) Immunoblotting of protein extracted from <i>N. benthamiana</i> leaves after transient assay, in presence or not of MG132 for 4 h. (D) Immunoblotting of protein extracted from <i>N. benthamiana</i> leaves after transient assay. Note the co-immunoprecipitation (Co-IP) of HA-HaRxL44 with GFP-MED19a in the presence of proteasome inhibitor.</p