Increases in antigen-specific IgG by PorB is dependent on TLR2 and MyD88.

Abstract

<p>(a) Concentration of IgG antibody to Ova in C57Bl/6 mice as measured by ELISA in serum on from day 42. WT C57Bl/6, TLR2 ^-/- and MyD88 ^-/- mice were vaccinated on days 0, 14 and 28 with Ova, Ova + PorB or sham (PBS). Vaccines that included PorB showed significantly less elevation in IgG over vaccines that did not include PorB in TLR2 ^-/- mice, and no detectable effect in MyD88 ^-/- mice. None of the mice had detectable antibodies to Ova prior to vaccination (data not shown). (b) OD of specific subtypes of anti-Ova IgG at a 1:50 dilution of serum as measured by ELISA. In WT mice, IgG1 and IgG2b were the dominant subtypes indicative of a Th2-type response. These in turn decreased in the TLR2 ^-/- mice, consistent with the total IgG data. Data represents one of two experiments. * p<0.05.</p