<p>Mice (3/group) transplanted i.p. with 3 × 10<sup>6</sup> ID8 cells 10 day earlier were injected i.p. twice at 4 days interval with 0.5 mg of anti-PD-1/CD137 mAb. Seven days after the second mAb injection, pooled splenocytes (5 × 10<sup>6</sup>) from 3 mice were incubated with 5 × 10<sup>5</sup> UV-irradiated ID8 cells for 4 days. The resultant splenocytes were then evaluated for antigen-specific CTL activity by CytoTox 96 Non-radioactive cytotoxicity assay using EL4 cells pulsed with H-2Db-restricted mesothelin or HPV-E7 peptide as target cells (A). The killing activity was also evaluated in the presence of anti-CD4, anti-CD8 or control antibody (B). Data were expressed as M±SEM of triplicate wells. </p
