Promotion of muscle lamellipodia development and interaction with neurons in <i>Abl</i> cultures.
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Abstract
<p>A–D) Samples of phase contrast images (100X) displaying lamellipodia from WT and <i>Abl</i> muscle cells in culture. (A) is from cultures incubated 7D, (B–D) are from cultures incubated 24 h–48 h. WT cultures from advanced embryos (stage 12) produce expanded lamellipodia when incubated at RT (A), whereas cultures derived from earlier embryos (stage 10) show muscle cells only after HT incubation, which display microspikes but rarely well extended lamellipodia (B). <i>Abl</i> cultures exhibit more abundant lamellipodia at stage 10 following incubation at RT (C) or HT (D). E) Example of neuron-muscle interactions in HT-incubated <i>Abl</i> cultures. Time lapse images (phase contrast, 40X) taken 24 hours (E1) and 39 hours (E2) after plating. Neuronal filopodia and muscle lamellipodia first approached each other (E1) and subsequently formed morphological connection (E2). Arrows indicate the interaction site. Scale bars, 10 µm.</p