pDC deficiency <i>in vivo</i> leads to altered cytokine responses.

Abstract

<p><b><i>A</i></b>, pDC depleted and the sham-treated mice (four mice/group), after <i>Cpn</i> infection were sacrificed 9 days postinfection and the lungs and draining (mediastinum) lymph nodes were collected. The draining lymph node (dLN) cells were cultured in the presence of SK-EB as described in <i>Materials and Methods</i>. Cytokine levels (IFN-γ, TNFα, IL-4 and IL-10) in 72-h dLN cell culture supernatants <i>(</i><b><i>A</i></b><i>)</i> and lung homogenates <i>(</i><b><i>B</i></b><i>)</i> were measured by ELISA. Data are presented as the mean ± SD of each group. Results of one of the three experiments with similar results are shown. *, <i>p</i><0.05, **, <i>p</i><0.01, and ***, <i>p</i><0.001; Student’s t test.</p