<i>Cpn</i> infection activated pDCs induce Treg IL-10 production <i>in vitro</i>.

Abstract

<p>pDC isolated from the lungs of <i>Cpn</i> infected (day 9 p.i.) and uninfected mice were co-cultured with CD4 T cells purified from <i>Cpn</i> immunized mice in the presence of SK-EB as described in the <i>Materials & Methods.</i> Following three days of co-culture, the cells were washed and analysed by flow cytometry. <b><i>A&B</i></b>, The cells were stained for intracellular Foxp3 expression. The analysis was performed on gated CD4+ cells. Representative histograms <i>(</i><b><i>A</i></b><i>)</i> are shown and the summary graph <i>(</i><b><i>B</i></b><i>)</i> provided. <b><i>C&D,</i></b> IL-10 production by Tregs; analysis was performed on CD4+Foxp3+ gated cells. Shown are representative dot plots <i>(</i><b><i>C</i></b><i>)</i> and a graphical summary <i>(</i><b><i>D</i></b><i>)</i> of the percentages of IL-10 producing Tregs. Data are shown as mean ± SD. Results of one representative (of three) experiment with five mice in each group are shown. *, p<0.05, **, p<0.01.</p