pDC deficiency leads to inflammatory type effector CD8 T and CD4 cell responses after <i>Cpn</i> infection.

Abstract

<p>Lung T cells as described in the legends to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083463#pone-0083463-g004" target="_blank">Figure 4</a> were analyzed by multi-color intracellular cytokine staining. Shown are representative flowcytometry dot plot images and summary graphs depicting the nature of cytokine responses by T cells. Analysis was performed on gated CD3+ CD8+ cells and CD3+CD4+ cells. Note increased inflammatory type-1 (IFNγ+TNFα+) CD8 <i>(</i><b><i>A</i></b><i>)</i> and CD4 T cells <i>(</i><b><i>B</i></b><i>)</i> and inflammatory Th2 type (IL-4+ TNFα+) CD4 T cells <i>(</i><b><i>C</i></b><i>)</i> in the lungs of pDC depleted mice compared to the control group mice. At least three independent experiments were performed and the data from one representative experiment is depicted. Data expressed as mean ± SD. *, p<0.05, and ***, p<0.001.</p