ADAR2 edits the specific nucleotide residues at the precursor and complementary antisense RNA transcripts of pri-miR-214 and pri-miR-122.

Abstract

<p>The Huh-7 cells were transfected with the plasmid constructs expressing either the sense (<b>S</b>) or the complementary antisense (<b>AS</b>) transcripts of pri-miR-214 (<b>A</b>) and pri-miR-122 (<b>B</b>), and then infected with lenti-ADARs as indicated. The RNA was extracted from each cell preparation for RT–PCR and subsequent direct sequencing analysis. The results shown here are representative, focusing on the regions covering the residues identified as the major editing sites (as revealed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081922#pone-0081922-g002" target="_blank">Figure 2</a>), including −37 and −28 for pri-miR-214 and −7 and +57 for pri-miR-122 (marked with arrows). The nucleotide residues showing editing events are marked with red asterisks.</p