<p>The frequencies of Tregs in the lungs and dLNs in pDC depleted and the sham-treated mice were analyzed following <i>Cpn</i> infection (day 9 p.i.). <b><i>A,</i></b> representative flowcytometry images show the percentages of CD4+Foxp3+ Tregs in the lungs and dLN<b>.</b> The absolute numbers of Tregs were depicted in the respective graphs. <b><i>B</i></b><i>,</i> Intracellular staining for IL-10; The dLN cells were cultured with SK-EB for 3 days and restimulated with PMA and ionomycin. The cells were stained first for surface markers, fixed, permeabilized and stained intracellularly for IL-10 as described in <i>Materials and Methods.</i> Shown are representative flowcytometry plots and the summary graphs for IL-10 production by Tregs (gated on CD4+Foxp3+CD8− cells). Data expressed as mean ± SD. Results are representative of three independent experiments with three mice in each group. **, p<0.01, ***, p<0.001.</p
