EMT markers are regulated upon ectopic expression of mutant p53, some of which are further enhanced by knockdown of endogenous wild-type p53 in MDCK cells.
<p><b>A</b>-<b>C</b>, Western blots were prepared with extracts from parental MDCK cells (lane 1), p53-KD MDCK cells (lane 2), MDCK cells in which a mutant p53 was ectopically expressed (lanes 3, 5) and MDCK cells in which a mutant p53 was ectopically expressed along with knockdown of endogenous wild-type p53 (lanes 4, 6). The blots were probed with antibodies against β-catenin (A), E-cadherin (A), Snail (B), Slug (B), Twist (B), c-Met (C) and actin (A-C). The protein levels of EMT markers were quantified and the ratios were labeled under the corresponding bands. <b>D</b>, Proposed model of mutant p53 in MDCK cell tubulogenesis. </p
