Examination of POU5F1 and MMP-2 by immunostaining and Western blot analyses.

Abstract

<p>(<b>A</b>) Immunostaining of POU5F1 and MMP-2 observed by confocal scanning microscopy. (<b>B</b>) Expression of stem cell transcription factor POU5F1 was detected by Western blot. (<b>C</b>) Immunohistochemical analysis of MMP-2 protein in tumor tissues formed by A549 LACSLCs and monolayer cells. Representative images of MMP-2 expression by IHC staining (20×) (<i>left panel</i>). Quantitative analysis of MMP-2 protein levels in tumor tissues formed by A549 LACSLCs and monolayer cells. All experiments were carried out at least in triplicates and the data are presented as the mean ± SEM. Student <i>t</i> test was performed to evaluate the difference.</p