Western blot analysis of dephosphorylated extracts obtained from dissected tissues of 2-month old mice using the Tau5 antibody.

Abstract

<p>(<b>A</b>) Analysis of wild-type (WT) mice; brain (bra), pancreas (pan), liver (liv), kidney (kid), muscle (mus), spleen (spl), testis (tes), and heart (hea). (<b>B</b>) Inclusion of tau knock-out (KO) tissue. Molecular weight and isoforms are indicated. (<b>C</b>) Western blot analysis of dephosphorylated brain extracts from 2-month old, 2-week old and P0 WT mice using the 3R- and 4R-specific antibodies RD3 and RD4, respectively. Note: The relatively intensities of RD3 and RD4 cannot be used to deduce the relative levels of the 3R and 4R isoforms. Instead, the Tau5 pattern is informative.</p