Correlation of HLA-DR associated peptides and peptide clusters measured by MAPPs to the amount of protein present in subvisible particles.

Abstract

<p>Linear regression analyses of the increase of the HLA-DR associated peptides and clusters as functions of the calculated amount of protein present in the subvisible particles. Left up: HLA-DR associated peptides of mAb1 vs protein amount in subvisible particles (r² = 0.994), left down: HLA-DR associated peptide clusters of mAb1 vs protein amount in subvisible particles (r² = 0.993), right up: HLA-DR associated peptides of mAb2 vs protein amount in subvisible particles (r² = 0.86), right down: HLA-DR associated peptide clusters of mAb2 vs protein amount in subvisible particles (r² = 0.943). HS: aggregates generated by heat and shake stress; FT: aggregates generated by freeze and thaw stress, S: aggregates generated by shear stress mAb1: monoclonal antibody 1, mAb2: monoclonal antibody 2, 1: stress level 1, 2: stress level 2. For further details, please, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086322#s4" target="_blank">Materials and Methods</a>.</p