IFN response in mice infected with the TURH influenza A virus strain.

Abstract

<p><b>A:</b> Immunofluorescence detection of TURH influenza A virus in liver sections from Mx-IFNAR1<sup>0/0</sup> and Mx-IFNLR1<sup>0/0</sup> mice infected for 48 hours or mock-infected, using anti-influenza A virus antibody (Red) and Hoechst staining of nuclei (blue). Results are representative of two independent experiments. <b>B–C:</b> RT-qPCR analysis of influenza A virus replication in the liver (B) and lungs (C) of Mx-IFNAR1<sup>0/0</sup> and Mx-IFNLR1<sup>0/0</sup> mice. Results are expressed as influenza A virus cDNA copies per 10<sup>5</sup> copies of β-actin cDNA. <b>D–E:</b> RT-qPCR analysis of <i>Oasl2</i> expression in the liver (D) and lungs (E) of Mx-IFNAR1<sup>0/0</sup> and Mx-IFNLR1<sup>0/0</sup> infected mice. Results are expressed as <i>mOasl2</i> cDNA copies per 10<sup>3</sup> copies of β-actin cDNA. <b>F–G:</b> IFN-β (F) and IFN-λ (G) production in the liver of infected mice. Results are expressed as <i>IFN</i> cDNA copies per 10<sup>5</sup> copies of β-actin cDNA. It is noteworthy that cells from IFNAR1<sup>0/0</sup> expressed lower basal levels of IFN, likely as a consequence of a disrupted positive feed-back loop for IFN expression in these mice.</p

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