Notch activation by DLL1 in POFUT1 deficient fibroblasts.

Abstract

<p>Western Blot analysis of cell lysates of NOTCH1 expressing HeLa cells (HeLaN1) co-cultured with POFUT1+/+ or POFUT1−/− fibroblasts or with CHO cells over-expressing DLL1 using the anti-Cleaved Notch1 (Val1744) antibody, which specifically detects the Notch1 intracellular domain (NICD) after S3 cleavage. Non-fucosylated DLL1 can efficiently activate Notch1.</p