Localization of endogenous DLL1 in PSM cells lacking POFUT1.

Abstract

<p>Confocal images of flat mounted PSMs from wild type and POFUT1 mutant E9.5 embryos (a-f). In wild type PSM cells, DLL1 was present almost exclusively on the cell surface and colocalized with Pan-Cadherin staining (c-c′). In POFUT1 mutant PSM cells most of the DLL1 protein was detected intracellularly in punctae or dots, reflecting significantly reduced colocalization with Pan-Cadherin (f-f′).</p