Analysis of human eCSF nanovesicles.

Abstract

<p>(<b>A</b>) Western blots for CD63, CD81, and HSP70 on purified human eCSF nanovesicles. (<b>B</b>) Pathscan analysis of human eCSF nanovesicles. All except CC3 are statistically above background at p<0.05. n.s., not significant. (<b>C</b>) Rank expression of human microRNAs based on microarray expression levels. (<b>D</b>) Differentially expressed microRNAs identified by Significant Analysis of Microarray (SAM) are indicated and represented in the heat map for human nanovesicles (left) and rat nanovesicles (right). Expression values range from low (bright green) to intermediate (black) to high (red). (<b>E</b>) Bioinformatic analysis of microRNA interacting pathways identified in eCSF purified human nanovesicles. Red microRNAs: 16-fold enriched (not shared with rat microRNAs); pink microRNA: 16-fold enriched and shared with rat; black microRNAs: 4-fold-enriched in humans and 16-fold enriched in rats. N = 4 humans. Error bars: SEM.</p