microRNA analysis of rat eCSF nanovesicles.

Abstract

<p>(<b>A</b>) Heat map of microRNA microarrays from eCSF purified nanovesicles. The lighter the color (yellow) indicates higher expression whereas the darker the color (dark purple) is an indication of absence of expression. Values range from (log₂) 0.64 (bottom) to (log₂) 15.4 (top). The top 24 enriched microRNAs are listed on the right. (<b>B</b>) Rank expression of microRNAs based on microarray expression levels. (<b>C</b>) Quantitative (q) RT-PCR of exosomal RNA using selective exosomal microRNA primers or lacking primers (Neg CTL: negative control). <u>Bottom</u>: Corresponding end-point RT-PCR. (<b>D</b>) Bioinformatic analysis of microRNA interacting pathways. N = 4 litters of rats.</p