LysoSensor Green DND-189 and DND-153 labeling of <i>E. chaffeensis</i>-infected DH82 cells.
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Abstract
<p>(A and D): The confocal images containing DAPI-stained nuclei and <i>E. chaffeensis</i> morulae (blue); (B): LysoSensor Green DND-153-labeled infected DH82 cells; (C): merged image of A and B; (E): LysoSensor Green DND-189-labeled infected DH82 cells (green); (F): merged image of D and E; (G): DAPI-stained uninfected DH82 cells; (H): LysoSensor probe-labeled uninfected DH82 cells; (I): merged image of G and H. Arrows indicate <i>E. chaffeensis</i> morulae. These results were confirmed in three independent labeling experiments.</p