<p>Rats were given a normal diet or a high-fat diet with or without 0.25, 0.5, or 1 g/kg EUE for 10 weeks, and serum and livers were harvested. Liver tissues were loaded with 5 µM dihydroethidium and fluorescence image acquisition was performed (A). Liver tissue was subjected to lipid peroxidation assay (B), caspase-3 activity assay (C), and immunoblotting with antibody against caspase-3, -9, or β-actin (D). Serum levels of AST and ALT were <b>measured (E)</b>. Following subcellular fractionation, immunoblotting with antibody against BAX, t-Bid, PDI, COX II, or LAMP-1 was performed (F). <sup>*</sup><i>p</i><0.05, significantly different from high-fat diet. HFD; high fat diet, EUE<i>; Eucommia ulmoides Oliver extract.</i></p
