<p>For Quantitative RT-PCR analysis, 8-day-old seedlings were pre-treated with 100 µM of salicylic acid for 24 h and then incubated in 1 µM flg22 solution for 1 h. <i>ACT2 </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088951#pone.0088951-Qiu1" target="_blank">[74]</a> was used as a control. Data represent SD. All quantitative gene expression measurements were performed using technical triplicate and biological duplicates. Differential letter types indicated significant differences (α = 0.05) by one-way ANOVA and Tukey HSD test of comparisons between plant genotypes with individual treatment.</p
