Cytokine production of human memory CD4⁺ T cells in response to <i>P. aeruginosa</i>.

Abstract

<p><b>A–C</b> Sorted human memory CD4⁺ T cells from healthy volunteers were co-cultured either with dendritic cells that had been infected with <i>P. aeruginosa</i> (DCs +) or with the supernatant from such infected DCs (DC −). Levels of IL-17A (<b>A</b>), IL-22 (<b>B</b>) and IFN-γ (<b>C</b>) were measured in supernatants after 6 days. DCs were infected at a MOI of 60 before culturing with T cells. Columns show the mean of triplicate determinations; error bars are ±1 standard error of mean. Results are representative of experiments in three separate individuals. <b>D–F</b>, secreted cytokine levels following DC infection at a variety of different MOIs. Each point represents a value from an individual healthy individual.</p