Cell proliferation of memory CD4⁺ T cells following co-culture with infected dendritic cells (DCs).

Abstract

<p>(<b>A</b>) Memory CD4⁺ T cells loaded with proliferation dye were co-cultured with unstimulated DCs, DCs infected with PA103ΔUΔT MOI 30 (PA103), or DCs infected with Yorkhill 5 MOI 30 (Yorkhill 5). Panels show the distribution of the proliferation dye fluorescence in the cell population after 6-days; inset figures show the percentage of the initial population that has undergone one or more cell divisions. (<b>B</b>) Patterns of cytokine expression by non-proliferating CD4⁺ T cells following 6-days of culture with DCs infected with Yorkhill 5. (<b>C</b>) Patterns of cytokine expression by CD4⁺ T cells proliferating in response to culture with DCs infected with Yorkhill 5. Numbers in plot represent per cent cells in each quadrant. The experiment was repeated in 3 independent individuals with the same result.</p