Cytokine production by human memory CD4⁺ T cells in healthy controls and cystic fibrosis.

Abstract

<p>Human memory CD4⁺ T cells were exposed to unstimulated DCs or DCs infected with the laboratory strains (PA103) or clinical <i>Pseudomonas aeruginosa</i> strains (Yorkhill). After 6 days of co-culture levels of (<b>A</b>) IL-17, (<b>B</b>) IL-22 and (<b>C</b>) IFN-γ were measured. Each point represents the mean of triplicate wells and is the result from one individual. The different bacterial strains used are: ♦, unstimulated; ○, PA103 ΔpcrV; •, PA103ΔUΔT; ▴, YH1; ▪, YH2; ×, YH5. The line indicates the median value. Differences between infection conditions (<b>A</b>–<b>C</b>) were evaluated by a Kruskal-Wallis test all of which had p values <0.0009). Differences between unstimulated and infected conditions were then tested using Dunn's multiple comparison test. ** significant p<0.01 and *** significant p<0.001.</p