<p>B6 mice were infected with LCMV Armstrong or left uninfected. After 30 days, mice were infected with <i>L. major</i>. After 4 weeks, <i>L. major</i> infected skin was taken and incubated with a pool of 20 LCMV derived peptides and BFA for 6 hours before staining for surface and intracellular proteins. Representative plots (A) and pooled data (B) are shown. Data are representative to 2 independent experiments (n = 5 per group). P14 T cells were transferred into B6 mice and the next day a group was infected with LCMV. After 30 days, GFP+ OTI T cells were transferred into all groups and mice were infected with <i>L. major</i>-OVA (C). After 4 weeks, <i>L. major</i> infected skin was harvested and incubated with BFA for 5 hours. The samples were analyzed for the presence of P14 cells or OTI cells (D) and numbers of each were calculated (E). The OTI or P14 cells from the same infected ear of an LCMV immune <i>L. major</i> infected mouse were also incubated with BFA, monensin, and CD107a antibody then stained for additional surface and intracellular proteins (F). Data are representative of four independent experiments (n = 4–5 per group). Percentages are shown as mean ± SEM.</p
