<p>B6 mice received CD45.1+ P14 cells (P14 chimeras) and were infected with LCMV for 5 days (effector time point) or 30 days (memory time point). At the indicated time post LCMV infection, splenocytes were harvested from naïve P14 mice or P14 chimeras and the numbers of P14 CD8 T cells were quantified. Equal numbers (5×10<sup>5</sup>) of P14 CD8 T cells were transferred into congenically marked B6 mice that had been infected with <i>L. major</i> for 2 weeks (A). After 36 hours, spleens (B), infected ears (C), and contralateral uninfected ears (D) were harvested from the recipients and P14 frequency was analyzed by flow cytometry (E). P14 CD8 T cells present in the spleen (F) or infected ear (G) were incubated with BFA alone for 5 hours and analyzed for production of gzmB and IFN-γ. The relative mean fluorescence intensity for each was calculated (H). Data are representative of two independent experiments (n = 3–5 per group).</p
