Tissue-homing characteristics of Pseudomonas aeruginosa-specific memory Th22 and Th17 cells.

Abstract

<p><b>A–C</b> Human memory CD4⁺ T cells were sorted into CCR6-depleted (CCR6 negative) and CCR6-enriched (CCR6 positive) populations before being exposed to dendritic cells (DCs) infected with Yorkhill 5 MOI30 (<b>A</b>), DCs infected with PA103 ΔUΔT MOI30 (<b>B</b>), or stimulated with anti-CD3 and anti-CD28 which acted as a positive control by polyclonal T cell stimulation (<b>C</b>). Plots show CD4⁺ IFN-γ negative T cells after 6-days of co-culture. <b>D</b> Memory CD4⁺ T cells were cultured with DCs infected with PA103 ΔpcrV MOI30. Pseudomonas-specific memory CD4⁺ T cells were analysed for expression of intracellular IL-22 or IL-17 as shown together with the skin-homing chemokine receptors CCR4, CCR10 and CCR9 as indicated. Numbers in each plot represent per cent cells in each quadrant. Results are representative of those seen in three independent individuals.</p