Quantitative real-time PCR assays from experimental mice for the following ileal mRNA transcripts: (A) cationic amino acid transporter (CAT-1); (B) arginase 1; (C) Toll-like receptor 9 (TLR9); and (D) Inducible nitric oxide synthase (iNOS).
- Publication date
- Publisher
Abstract
<p>Experimental mice were challenged by a compounded malnutrition and <i>Cryptosporidium parvum</i> insult and samples were harvested on day 7 post-<i>C. parvum inoculum</i>. Wild-type, APOE knockout, and APOE targeted replacement mice (APOE 3/3 TR and APOE 4/4 TR) were orally inoculated with 10<sup>7</sup>- unexcysted oocysts diluted in 100 µl of PBS. Groups have at least 4 per groups and the results are shown as mean ±SEM and expressed after β-actin normalization.</p
