Computational cost of fitting in time - and Legendre - domain.

Abstract

<p>Dots and asterisks indicate the computational cost in Legendre - and time - domain, respectively, on a logarithmic scale. Black and red curves refer to whether (red) or not (black) a system response function was taken into account. Each point or asterisk is the average duration of computations. As the analysis in the time - domain is fastest when using the Fast Fourier Transform, we choose the sample sizes to be powers of , starting with . Inset, left, FLIM image of a mouse hippocampus neuron probed for oxidative stress using the hydrogen peroxide sensor HyPer <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090500#pone.0090500-Belousov1" target="_blank">[13]</a> and, right, fluorescence lifetime function for one pixel in the middle of the cell (150 samples, data courtesy K. Kizina and M. Müller, CNMPB, Göttingen).</p

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