Identification and classification of differentially expressed proteins between <i>02</i> and <i>8213</i>.

Abstract

<p>(A) Venn diagram showing 20 up-regulated and 5 down-regulated proteins among 48 identified proteins comparing <i>02</i>-HS with <i>02</i>-NS. Functional classification of those 25 heat stress induced proteins is shown on the bottom. (B) Heat-map is shown to indicate relative protein abundance among the 4 samples. The abundance of each protein spot in <i>02</i>-NS was given a reference value of 1.0, the abundance of that spot from <i>02</i>-HS, <i>8213</i>-NS and <i>8213</i>-HS were transformed into relative value. The different colors correspond to the values of protein level changes as indicated by the bar at the bottom of the heat map. Venn diagrams present the two-step filtering process leading to 4 candidates to further select out the more important candidate proteins for functional studies (see text). The four asterisks mark the proteins that are upregulated by heat-stress in thermotolerant strain <i>02</i>, not upregulated by heat stress in thermo-sensitive strain <i>8213,</i> and expressed at lower levels in thermo-sensitive strain <i>8213</i> than in the thermotolerant strain <i>02</i> with or without heat-stress. <i>02</i>-NS: <i>02</i> non-stressed (23°C/24 h); <i>02</i>-HS: <i>02</i> heat-stressed (33°C/24 h); <i>8213</i>-NS: <i>8213</i> non-stressed (23°C/24 h); <i>8213</i>-HS: strain <i>8213</i> heat-stressed (33°C/24 h).</p

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