MAP4K4 promotes lamellipodium formation and enhances ERM accumulation in lamellipodia.

Abstract

<p><b>A</b>) Confocal IFA of endogenous MAP4K4 (green), F-actin (red) and TaSP (blue) in cells transfected with siControl or siMAP4K4. Bar diagram shows quantification of percent cells displaying a large, single lamellipodium in the absence (black) or presence (white) of 25 ng/ml TNFα. C: siControl; 1: siMAP4K4_1; 2: siMAP4K4_2 <b>B</b>) IFA of total ERM (anti-ERM ab) proteins and ERM proteins phosphorylated on C-terminal threonine (anti-pERM ab) in cells seeded on Fn. <b>a</b> and <b>b</b> show 4× magnifications of boxed areas for ERM (<b>a</b>) and pERM (<b>b</b>) distribution. F-actin is in red, ERM and pERM in green, DNA in blue. <b>C</b>) IFA of ERM localization in siControl or siMAP4K4 transfected cells. IFA as in B, <b>c</b> and <b>d</b> show 4× magnifications of boxed areas for ERM accumulation in lamellipodia of siControl (<b>c</b>) and or siMAP4K4 transfected cells (<b>d</b>). See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004003#ppat.1004003.s006" target="_blank">Figure S6</a>.</p