The ATDCs expressed higher levels of IL-6, TGF-β and IL-23 than SPDCs.

Abstract

<p>The ATDCs and SPDCs of HFD mice were isolated by microbeads method, and then incubated them with PMA and ionomycin at 37°C for 4 hours. mRNA levels of IL-6, TGF-β, IL-23p19, IL-12p40 and IL-12p35 in the ATDCs and SPDCs were examined by qRT-PCR method. The concentration of IL-6, IL-23 in the supernatant were examined by ELISA method. Data are presented as mean ± SD of 6 samples pooled from three independent experiments. Student's t-test was used for the statistical analysis. **<i>p</i><0.01, *<i>p</i><0.05.</p