There are more DCs in HFD mice AT than ND mice AT.

Abstract

<p>(A) CD11c⁺ DCs expression in HFD mice AT. CD11c⁺ DCs in HFD mice AT were analyzed using flow cytometry. And proportions of CD11c⁺ DC in the AT of HFD mice (n = 6) and ND mice (n = 6). (B) Morphology of DC-like cells in HFD mice AT under transmission electron microscope. (C) The CD11c⁺ DCs in HFD mice AT were negative for CD3 and B220. Open histograms indicate isotype control, numbers in histograms represent the geometric mean fluorescence. (D, E) The CD11c⁺ DCs in HFD mice AT are different from macrophages. (D) The phagocytosis ability of ATDCs and macrophages assessed by incubating with HcRed-expressed <i>E.coli</i> at 37°C for 4 hours. Numbers in histograms represent the geometric mean fluorescence. (E) The CD11c⁺ DCs and macrophages from HFD mice spleen or adipose tissues were stained with specific mAb against F4/80, cells were gated on the CD11c⁺ population, and numbers in histograms represent the geometric mean fluorescence of the gated cells. All data are shown as the mean ± SD of 6 samples pooled from three independent experiments. Student's t-test was used for the statistical analysis. ***<i>p</i><0.001.</p