Validation of differentially expressed genes by real-time PCR.
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Abstract
<p>Comparison of RNA-seq and real-time PCR measures of changes in expression of <i>eutD</i> (encoding phosphotransacetylase), <i>rpmE</i> (50S ribosomal protein L31), <i>acpP</i> (acyl carrier protein), <i>tte0510</i> (hypothetical protein), <i>tte2654</i> (hypothetical protein), <i>tte0106</i> (hypothetical protein), <i>dnaA</i> (chromosome replication initiator DnaA), <i>tte1002</i> (hypothetical protein), <i>pspf4</i> (a transcriptional regulator), and <i>cspC</i> (cold shock protein). All data are shown as means ± SD (n = 3).</p