Identification of tumors harboring the <i>ALK^F1174L</i> mutation in <i>MYCN</i>-driven transgenic mice with intrinsic susceptibility MRI.

Abstract

<p><b>A</b>) Anatomical transverse T₂*-weighted MR images acquired at TE = 6.8 and 15.6 ms from representative Th-<i>ALK^F1174L</i>/Th-<i>MYCN</i> and Th-<i>MYCN</i> mice with abdominal neuroblastoma during initial air breathing, and at TE = 15.6 ms after 5 minutes of continuous inhalation of 100% oxygen. <b>B</b>) Corresponding parametric tumor transverse relaxation rate R₂* maps calculated during initial air breathing and after 3 minutes of continuous inhalation of 100% oxygen. <b>C</b>) Resulting parametric tumor ΔR₂*_oxygen-air (R₂*_oxygen−R₂*_air) maps. <b>D</b>) Tumor R₂* during initial air breathing, <b>E</b>) tumor R₂* after 5 minutes of breathing 100% oxygen, and <b>F</b>) tumor ΔR₂*_oxygen-air (R₂*_oxygen−R₂*_air) were determined from Th-<i>ALK^F1174L</i>/Th-<i>MYCN</i> and Th-<i>MYCN</i> mice with abdominal neuroblastoma. Individual data points represent the mean of the median values determined from all three imaging slices for each animal, as well as the mean ±1 s.e.m, p, Student's 2-tailed unpaired t-test with a 5% level of significance.</p