Effects of S100A7 knockdown on MMP and mitochondrial homeostasis.

Abstract

<p>(<b>A</b>) Expression of S100A7 mRNA after siRNA transfection. HaCaT cells were transfected with S100A7 siRNA using lipofectamin 2000 for 48 h. Total RNA was extracted and subjected to quantitative RT-PCR. The amount of S100A7 mRNA relative to β-actin is shown. (<b>B</b>) S100A7-siRNA HaCaT cells were treated for 24 h with LPS at 100 and 1000 ng/ml. The MMPs were assayed. (<b>C</b>, <b>D</b>) The mitochondrial dynamics-related proteins DRP1 and Mfn2 and the autophagy-related protein LC3B were detected by Western blot (<b>C</b>, Western blot images; <b>D</b>, statistical results). Values are means ± SEM. Statistical significance is indicated (^#<i>P</i><0.05, *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001). cont, control; veh-si, vehicle-siRNA; veh-si-100 or veh-si-1000, vehicle-siRNA cells with 100 or 1000 ng/ml LPS treatment; pso-si, S100A7-siRNA; pso-si-100 or pso-si-1000, S100A7-siRNA HaCaT cells with 100 or 1000 ng/ml LPS treatment.</p