Effects of metallic nickel particles on p53 transcription activity and protein levels.

Abstract

<p>JB6 cells, stably transfected with p53 luciferase reporter plasmid, were seeded onto a 24-well plate and incubated overnight. Cells were treated with/without metallic nickel nano- or fine particles for 24 h. The p53 transcription activity and protein levels were measured by the luciferase activity assay (A) and western-blot (B), respectively. Metallic nickel nanoparticles caused a greater decrease of p53 transcription activity and protein levels than fine particles. * <i>P</i><0.05 <i>versus</i> control. TPA (20 ng/ml) or UVB (4 kJ/m²) were set as a positive control.</p