AD1 contributes to Nrf1-mediated transactivation of ARE-driven reporter genes.

Abstract

<p>(<b>A</b>) The <i>left</i> schematic illustrates the relative positions of PEST1, Neh2L, CPD and Neh5L within AD1. The DIDLID/DLG element and the ETGE motif are situated in Neh2L, which overlaps PEST1. The <i>right</i> panel shows that discrete regions of AD2 make different contributions to Nrf1 activity. Cells were transfected with the indicated expression plasmids, along with that for <i>GSTA2-</i>6×ARE-Luc reporter construct. After recovery in 5.5 mM-glucose medium, the cells were cultured for a further 18 h in glucose-free or 25 mM-glucose-containing medium, before luciferase activity was measured. Significant decreases (**p<0.001, n = 9) relative to wild-type Nrf1 activity are indicated. (<b>B</b> and <b>C</b>) These samples were also subjected to western blotting and cross-reacting polypeptides were visualized by ECL.</p