<p>(<b>A</b>) Control (pLKO) or SNX17 knockdown N2a cells expressing ApoER2 were subjected to subcellular fractionation to isolate endosomal compartments by a discontinuous sucrose gradient. Samples of the different fractions were resolved in Tris/Tricine gels and analyzed by western blot. The input corresponds to 5% of the lysate. EE/RE: early endosome/recycling endosome; LE: late endosome; fractions were free of endoplasmic reticulum (RAP) or Golgi (γ-Adaptin) contamination. (<b>B</b>) Quantification of ApoER2 in different compartments normalized by the Input. *p<0.05.</p
