<p>DIV 4 cortical mouse neurons were infected with the a lentiviral system expressing the parental plasmid pLKO (Control) or SNX17 shRNA (shSNX17) at MOI 1; three days after infection, the cells were incubated with reelin-containing medium. Next, cells were lysed and analyzed by western blot. Dab1 was immunoprecipitated (IP) with an anti-Dab1 antibody and analyzed by western blot using an anti-phosphotyrosine antibody. The Dab1 downstream targets AKT and GSK3β were also analyzed by western blot. For all of the proteins analyzed, the reelin-induced phosphorylation was reduced in the SNX17 knockdown cells.</p
