Tol2-mediated transgenesis with pTol2{rps9::egfp} yields robust, ubiquitous expression of EGFP in G₀ animals.

Abstract

<p>(<b>A, B</b>) pTol2{rps9::egfp}^frkt868-injected animals co-injected with <i>tol2</i> mRNA (A) show significantly stronger EGFP fluorescence (green arrows) than those without transposase (B) (ae: autofluorescence around the adult eyes). (<b>C, D</b>) Stable ubiquitous EGFP expression (green arrows) two months after co-injection of donor DNA and <i>tol2</i> mRNA (C) compared to a non-injected animal (D). Labels indicate autofluorescence of the jaws (j) as well as the iridophore pigments (p) in both the head and trunk.</p